2.43
1.65
1.98
0.73
1.88
1.81
2.43
2.2 Zat standar anu dianggo dina kurva kalibrasi distribusi massa molekul relatif: insulin, mikopeptida, glisin-glisin-tirosin-arginin, glisin-glisin-glisin
3 Instrumen sareng peralatan
23.2
21.4
22.2
16.1
22.3
20.8
23.9
27.5
Sacara umum, proporsi asam amino dina produk Sustar langkung luhur tibatan dina produk Zinpro.
Bagian 8 Pangaruh panggunaan
Pangaruh tina rupa-rupa sumber mineral renik kana kinerja produksi sareng kualitas endog hayam petelur dina période petelur akhir
Prosés Produksi
Téhnologi khelasi anu ditujukeun
Téhnologi émulsifikasi geser
Téhnologi semprot & pangeringan tekanan
Téhnologi pendinginan & dehumidifikasi
Téhnologi kontrol lingkungan anu canggih
Lampiran A: Métode pikeun Nangtukeun distribusi massa molekul relatif péptida
Adopsi standar: GB/T 22492-2008
1 Prinsip Tés:
Ieu ditangtukeun ku kromatografi filtrasi gél kinerja tinggi. Hartina, nganggo pangisi porous salaku fase stasioner, dumasar kana bédana ukuran massa molekul relatif komponén sampel pikeun pamisahan, anu dideteksi dina beungkeut péptida tina panjang gelombang serapan ultraviolét 220 nm, nganggo parangkat lunak pamrosésan data khusus pikeun nangtukeun distribusi massa molekul relatif ku kromatografi filtrasi gél (nyaéta, parangkat lunak GPC), kromatogram sareng datana diolah, diitung pikeun kéngingkeun ukuran massa molekul relatif péptida kacang kedelai sareng rentang distribusi.
2. Réagen
Cai ékspériméntal kedah nyumponan spésifikasi cai sekundér dina GB / T6682, panggunaan réagen, kecuali pikeun katangtuan khusus, sacara analitis murni.
2.1 Réagen kalebet asetonitril (sacara kromatografi murni), asam trifluoroasetat (sacara kromatografi murni),
2.2 Zat standar anu dianggo dina kurva kalibrasi distribusi massa molekul relatif: insulin, mikopeptida, glisin-glisin-tirosin-arginin, glisin-glisin-glisin
3 Instrumen sareng peralatan
3.1 Kromatograf Cair Kinerja Tinggi (HPLC): stasiun kerja atanapi integrator kromatografi kalayan detektor UV sareng perangkat lunak pangolah data GPC.
3.2 Unit filtrasi sareng degassing vakum fase gerak.
3.3 Neraca éléktronik: nilai ukur 0.000 1g.
4 Léngkah-léngkah operasi
4.1 Kaayaan kromatografi sareng ékspérimén adaptasi sistem (kaayaan rujukan)
- 4.1.1 Kolom kromatografi: TSKgelG2000swxl300 mm×7.8 mm (diaméter jero) atawa kolom gél séjénna nu sarua jenisna kalawan kinerja nu sarupa nu cocog pikeun nangtukeun protéin jeung péptida.
- 4.1.2 Fase gerak: Asetonitril + cai + asam trifluoroasetat = 20 + 80 + 0,1.
- 4.1.3 Panjang gelombang deteksi: 220 nm.
- 4.1.4 Laju aliran: 0,5 mL/mnt.
- 4.1.5 Waktos deteksi: 30 menit.
- 4.1.6 Volume suntikan sampel: 20μL.
- 4.1.7 Suhu kolom: suhu kamar.
- 4.1.8 Supados sistem kromatografi nyumponan sarat deteksi, ditetepkeun yén dina kaayaan kromatografi di luhur, efisiensi kolom kromatografi gél, nyaéta, jumlah téoritis pelat (N), henteu kirang ti 10000 anu diitung dumasar kana puncak standar tripeptida (Glisin-Glisin-Glisin).
- 4.2 Produksi kurva standar massa molekul relatif
- Larutan standar péptida massa molekul relatif anu béda-béda di luhur kalayan konsentrasi massa 1 mg/mL disiapkeun ku cara cocogkeun fase gerak, dicampur dina proporsi anu tangtu, teras disaring ngaliwatan mémbran fase organik kalayan ukuran pori 0,2 μm ~ 0,5 μm sareng diinjeksikeun kana sampel, teras kromatogram standar diala. Kurva kalibrasi massa molekul relatif sareng persamaanna diala ku cara ngaplot logaritma massa molekul relatif ngalawan waktos ingetan atanapi ku régrési linier.
4.3 Perlakuan sampel
Timbang sacara akurat 10 mg sampel dina labu ukur 10 mL, tambahkeun saeutik fase gerak, kocok ultrasonik salami 10 menit, supados sampel leyur sareng dicampur, diéncérkeun ku fase gerak kana timbangan, teras disaring ngalangkungan mémbran fase organik kalayan ukuran pori 0,2 μm ~ 0,5 μm, sareng filtratna dianalisis numutkeun kaayaan kromatografi dina A.4.1.
- 5. Itungan distribusi massa molekul relatif
- Saatos nganalisis larutan sampel anu disiapkeun dina 4.3 dina kaayaan kromatografi 4.1, massa molekul relatif sampel sareng rentang distribusina tiasa diala ku cara ngagantikeun data kromatografi sampel kana kurva kalibrasi 4.2 nganggo parangkat lunak pangolah data GPC. Distribusi massa molekul relatif tina péptida anu béda tiasa diitung ku metode normalisasi daérah puncak, numutkeun rumus: X=A/A total×100
- Dina rumusna: X - Fraksi massa péptida massa molekul relatif dina total péptida dina sampel, %;
- A - Puncak wewengkon péptida massa molekul relatif;
- Total A - jumlah wewengkon puncak unggal péptida massa molekul relatif, diitung nepi ka hiji tempat desimal.
- 6 Kabisa diulang
- Beda absolut antara dua panangtuan mandiri anu diala dina kaayaan kabisaulangan teu kedah ngaleuwihan 15% tina rata-rata aritmatika tina dua panangtuan éta.
- Lampiran B: Métode pikeun Nangtukeun Asam Amino Bébas
- Diadopsi standar: Q/320205 KAVN05-2016
- 1.2 Réagen sareng bahan
- Asam asetat glasial: murni sacara analitis
- Asam perklorat: 0,0500 mol/L
- Indikator: indikator kristal ungu 0,1% (asam asetat glasial)
- 2. Nangtukeun asam amino bébas
Sampel dikeringkeun dina suhu 80°C salami 1 jam.
Simpen sampel dina wadah garing supados niiskeun sacara alami dugi ka suhu kamar atanapi tiiskeun dugi ka suhu anu tiasa dianggo.Timbang kira-kira 0,1 g sampel (akurat dugi ka 0,001 g) kana labu kerucut garing 250 mL.Gancang teraskeun kana léngkah salajengna pikeun nyegah sampel nyerep Uap cai di sakurilingna.Tambahkeun 25 mL asam asetat glasial teras aduk rata salami teu langkung ti 5 menit.Tambahkeun 2 tetes indikator kristal unguTitrasi ku 0,0500 mol/L (±0,001) larutan titrasi standar asam perklorat nepi ka larutan robah ti wungu ka titik ahir.
Catet volume larutan standar anu dikonsumsi.
- Laksanakeun tés kosong dina waktos anu sami.
- 3. Itungan sareng hasilna
- Eusi asam amino bébas X dina réagen dinyatakeun salaku fraksi massa (%) sareng diitung dumasar kana rumus: X = C × (V1-V0) × 0,1445/M × 100%, dina rumus ieu:
- C - Konsentrasi larutan asam perklorat standar dina mol per liter (mol/L)
- V1 - Volume anu dianggo pikeun titrasi sampel nganggo larutan asam perklorat standar, dina mililiter (mL).
- Vo - Volume anu dianggo pikeun titrasi blanko nganggo larutan asam perklorat standar, dina mililiter (mL);
M - Massa sampel, dina gram (g).
| 0,1445: Massa rata-rata asam amino sarua jeung 1,00 mL larutan asam perklorat standar [c (HClO4) = 1,000 mol / L]. | 4.2.3 Larutan titrasi standar serium sulfat: konsentrasi c [Ce (SO4) 2] = 0,1 mol/L, disiapkeun numutkeun GB/T601. | |
| Adopsi standar: Q/70920556 71-2024 | 1. Prinsip panangtuan (Fe salaku conto) | Kompleks beusi asam amino mibanda kalarutan anu handap pisan dina étanol anhidrat sareng ion logam bébas leyur dina étanol anhidrat, bédana kalarutan antara duanana dina étanol anhidrat dianggo pikeun nangtukeun laju khelasi kompleks beusi asam amino. |
| Dina rumus: V1 - volume larutan standar cerium sulfat anu dikonsumsi pikeun titrasi larutan uji, mL; | Étanol anhidrat; sésana sami sareng klausa 4.5.2 dina GB/T 27983-2011. | 3. Léngkah-léngkah analisis |
| Laksanakeun dua uji coba sacara paralel. Timbang 0,1 g sampel anu dikeringkeun dina suhu 103 ± 2 ℃ salami 1 jam, akurasi dugi ka 0,0001 g, tambahkeun 100 mL étanol anhidrat pikeun ngaleyurkeun, saring, sésa saring dikumbah ku 100 mL étanol anhidrat sahenteuna tilu kali, teras pindahkeun sésa kana labu kerucut 250 mL, tambahkeun 10 mL larutan asam sulfat numutkeun klausa 4.5.3 dina GB/T27983-2011, teras laksanakeun léngkah-léngkah ieu numutkeun klausa 4.5.3 "Panaskan pikeun ngaleyurkeun teras antepkeun tiis" dina GB/T27983-2011. Laksanakeun uji kosong dina waktos anu sami. | 4. Nangtukeun kandungan total beusi | 4.1 Prinsip panangtuan sami sareng klausa 4.4.1 dina GB/T 21996-2008. |
4.2. Réagén & Larutan
| 4.2.1 Asam campuran: Tambahkeun 150mL asam sulfat sareng 150mL asam fosfat kana 700mL cai teras aduk rata. | 4.2.2 Larutan indikator natrium difenilamin sulfonat: 5g/L, disiapkeun numutkeun GB/T603. | 4.2.3 Larutan titrasi standar serium sulfat: konsentrasi c [Ce (SO4) 2] = 0,1 mol/L, disiapkeun numutkeun GB/T601. | |
| 4.3 Léngkah-léngkah analisis | Laksanakeun dua uji coba sacara paralel. Timbang 0,1 g sampel, akurat dugi ka 020001 g, lebetkeun kana labu kerucut 250 mL, tambahkeun 10 mL asam campuran, saatos leyur, tambahkeun 30 ml cai sareng 4 tetes larutan indikator natrium dianilin sulfonat, teras laksanakeun léngkah-léngkah ieu numutkeun klausa 4.4.2 dina GB/T21996-2008. Laksanakeun uji kosong dina waktos anu sami. | 4.4 Répréséntasi hasil | Kandungan beusi total X1 tina kompleks beusi asam amino dina hal fraksi massa beusi, nilai anu dinyatakeun dina %, diitung dumasar kana rumus (1): |
| X1=(V-V0)×C×M×10-3×100 | V0 - larutan standar cerium sulfat anu dikonsumsi pikeun titrasi larutan blanko, mL; | V0 - larutan standar cerium sulfat anu dikonsumsi pikeun titrasi larutan blanko, mL; | C - Konsentrasi sabenerna tina larutan standar cerium sulfat, mol/L5. Itungan kandungan beusi dina kelatKandungan beusi X2 dina kelat dina fraksi massa beusi, nilai anu dinyatakeun dina %, diitung dumasar kana rumus: x2 = ((V1-V2) × C × 0.05585)/m1 × 100 |
| Dina rumus: V1 - volume larutan standar cerium sulfat anu dikonsumsi pikeun titrasi larutan uji, mL; | V2 - larutan standar cerium sulfat anu dianggo pikeun titrasi larutan blanko, mL;nom1-Massa sampel, g. Anggo rata-rata aritmatika tina hasil panangtuan paralel salaku hasil panangtuan, sareng bédana absolut tina hasil panangtuan paralel henteu langkung ti 0,3%. | 0,05585 - massa beusi ferous anu dinyatakeun dina gram sarua jeung 1,00 mL larutan standar cerium sulfat C[Ce(SO4)2,4H20] = 1,000 mol/L.nom1-Massa sampel, g. Anggo rata-rata aritmatika tina hasil panangtuan paralel salaku hasil panangtuan, sareng bédana absolut tina hasil panangtuan paralel henteu langkung ti 0,3%. | 6. Itungan laju khelasiLaju khelasi X3, nilaina dinyatakeun dina %, X3 = X2/X1 × 100Lampiran C: Métode pikeun Nangtukeun Laju Khelasi Zinpro |
Diadopsi standar: Q/320205 KAVNO7-2016
1. Réagen sareng bahan
a) Asam asetat glasial: sacara analitis murni; b) Asam perklorat: 0,0500mol/L; c) Indikator: indikator kristal ungu 0,1% (asam asetat glasial)
2. Nangtukeun asam amino bébas
2.1 Sampel dikeringkeun dina suhu 80°C salami 1 jam.
2.2 Simpen sampel dina wadah garing pikeun niiskeun sacara alami dugi ka suhu kamar atanapi niiskeun dugi ka suhu anu tiasa dianggo.
2.3 Timbang kira-kira 0,1 g sampel (akurat dugi ka 0,001 g) kana labu kerucut garing 250 mL
2.4 Gancang teraskeun ka léngkah salajengna pikeun nyegah sampel nyerep Uap cai di sabudeureun.
2.5 Tambahkeun 25 mL asam asetat glasial teras aduk rata salami teu langkung ti 5 menit.
2.6 Tambahkeun 2 tetes indikator kristal ungu.
2.7 Titrasi ku 0,0500mol/L (±0,001) larutan titrasi standar asam perklorat nepi ka larutan robah ti wungu jadi héjo salila 15 detik tanpa ngarobah warna salaku titik ahirna.
2.8 Catet volume larutan standar anu dikonsumsi.
2.9 Laksanakeun tés kosong dina waktos anu sami.
- 3. Itungan sareng hasilna
- Katalan
- Physicochemical parameters
V1 - Volume anu dianggo pikeun titrasi sampel nganggo larutan asam perklorat standar, dina mililiter (mL).
Vo - Volume anu dianggo pikeun titrasi blanko nganggo larutan asam perklorat standar, dina mililiter (mL);
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Alamat: No.147 Jalan Qingpu, Kota Shouan, Kabupatén Pujiang, Kota Chengdu, Propinsi Sichuan, Cina
Telepon: 86-18880477902
Produk
Mineral renik anorganik
- Mineral renik organik
- Basa Swahili
- Layanan khusus
- Tautan gancang
Profil Perusahaan
| Application object | Suggested dosage (g/t full-value material) | Content in full-value feed (mg/kg) | Efficacy |
| Gujarati | Klik kanggo patarosan | © Hak Cipta - 2010-2025: Sadaya Hak Cipta Ditangtayungan. | Peta situs PILARIAN UTAMA Telepon |
| Telp. | 86-18880477902 | Jawa | Surélék |
| 8618880477902 | Cina | Perancis | |
| Bird | Cina | Perancis | Jerman Spanyol |
| Aquatic animals | Basa Jepang | Koréa | Basa Arab Yunani |
| Turki | Italia | ||
| Ruminant animal g/head day | January 0.75 | Basa Indonésia Afrikaans Swédia |
Polandia
- Basa Basque
- Katalan
- Physicochemical parameters
Hindi
Lao
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Shona
Basa Bulgaria
- Basa Cebuano
- This product is chemically stable and can significantly reduce its damage to vitamins and fats, etc. The use of this product is conducive to improving feed quality;
- The product is absorbed through small peptide and amino acid pathways, reducing the competition and antagonism with other trace elements, and has the best bio-absorption and utilization rate;
- Kroasia
Walanda
| Application object | Urdu Vietnam | Content in full-value feed (mg/kg) | Efficacy |
| Gujarati | Haiti | Hausa | Kinyarwanda Hmong Hungaria |
| Piglets and fattening pigs | Igbo | Jawa | Basa Kannada Khmer Kurdi |
| Kirgiz | Latin | ||
| Bird | 300～400 | 45～60 | Makedonia Melayu Malayalam |
| Aquatic animals | 200～300 | 30～45 | 1. Promote growth, improve feed conversion; 2. Improve anti-stress abolity, reduce morbidity and mortality. |
Basa Norwegia
- Basa Pashto
- Appearance: brownish-yellow granules
- Physicochemical parameters
Serbia
Sesotho
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Shona
Basa Sindhi
This product is an all-organic trace mineral chelated by a special chelating proces with pure plant enzymatic small molecule peptides as chelating substrates and trace elements;
Basa Swahili
Tajik
Tamil
Telugu
Thailand
| Application object | Urdu Vietnam | Content in full-value feed (mg/kg) | Efficacy |
| Basa Yiddish | Yoruba | Zulu | Kinyarwanda Oriya Turkmen |
| Uighur | 250～400 | 37.5～60 | 1. Improving the immunity of piglets, reducing diarrhea and mortality; 2. Improving palatability, increasing feed intake, increasing growth rate and improving feed conversion; 3. Make the pig coat bright and improve the carcass quality and meat quality. |
| Bird | 300～400 | 45～60 | 1. Improve feather glossiness; 2. improve the laying rate, fertilization rate and hatching rate of breeding eggs, and strengthen the coloring ability of egg yolk; 3. Improve anti-stress ability and reduce mortality; 4. Improve feed conversion and increase growth rate. |
| Aquatic animals | January 300 | 45 | 1. Promote growth, improve feed conversion; 2. Improve anti-stress abolity, reduce morbidity and mortality. |
| Ruminant animal g/head day | 2.4 | 1. Improve milk yield, prevent mastitis and foof rot, and reduce somatic cell content in milk; 2. Promote growth, improve feed conversion and improve meat quality. |
4. Manganese Amino Acid Chelate Feed Grade
- Product Name: Manganese Amino Acid Chelate Feed Grade
- Appearance: brownish-yellow granules
- Physicochemical parameters
a) Mn: ≥ 10.0%
b) Total amino acids: ≥ 19.5%
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
n=0, 1,2,...indicates chelated manganese for dipeptides, tripeptides, and tetrapeptides
Characteristics of Manganese Amino Acid Chelate Feed Grade
This product is an all-organic trace mineral chelated by a special chelating proces with pure plant enzymatic small molecule peptides as chelating substrates and trace elements;
This product is chemically stable and can significantly reduce its damage to vitamins and fats, etc. The use of this product is conducive to improving feed quality;
The product is absorbed through small peptide and amino acid pathways, reducing the competition and antagonism with other trace elements, and has the best bio-absorption and utilization rate;
The product can improve the growth rate, improve feed conversion and health status significantly; and improve the laying rate, hatching rate and healthy chick rate of breeding poultry obviously;
Manganese is necessary for bone growth and connective tissue maintenance. It is closely related to many enzymes; and participates in carbohydrate, fat and protein metabolism, reproduction and immune response.
Usage and Efficacy of Manganese Amino Acid Chelate Feed Grade
| Application object | Suggested dosage (g/t full-value material) | Content in full-value feed (mg/kg) | Efficacy |
| Breeding pig | 200～300 | 30～45 | 1. Promote the normal development of sexual organs and improve sperm motility; 2. Improve the reproductive capacity of breeding pigs and reduce reproductive obstacles. |
| Piglets and fattening pigs | 100～250 | 15～37.5 | 1. It is beneficial to improve immune functions, and improve anti-stress ability and disease resistance; 2. Promote growth and improve feed conversion significantly; 3. Improve meat color and quality, and improve lean meat percentage. |
| Bird | 250～350 | 37.5～52.5 | 1. Improve anti-stress ability and reduce mortality; 2. Improve laying rate, fertilization rate and hatching rate of breeding eggs, improve eggshell quality and reduce shell breaking rate; 3. Promote bone growth and reduce the incidence of leg diseases. |
| Aquatic animals | 100～200 | 15～30 | 1. Promote growth and improve its anti-stress ability and disease resistance; 2. Improve sperm motility and hatching rate of fertilized eggs. |
| Ruminant animal g/head day | Cattle 1.25 | 1. Prevent fatty acid synthesis disorder and bone tissue damage; 2. Improve reproductive capacity, prevent abortion and postpartum paralysis of female animals, reduce the mortality of calves and lambs, and increase the newborn weight of young animals. | |
| Goat 0.25 |
Part 6 FAB of Small Peptide-mineral Chelates
| S/N | F: Functional attributes | A: Competitive differences | B: Benefits brought by competitive differences to users |
| 1.52 | Selectivity control of raw materials | Select pure plant enzymatic hydrolysis of small peptides | High biological safety, avoiding cannibalism |
| 2 | Directional digestion technology for double protein biological enzyme | High proportion of small molecular peptides | More "targets", which are not easy to saturation, with high biological activity and better stability |
| 3 | Advanced pressure spray & drying technology | Granular product, with uniform particle size, better fluidity, not easy to absorb moisture | Ensure easy to use, more uniform mixing in complete feed |
| Low water content (≤ 5%), which greatly reduces the influence caused by vitamins and enzyme preparations | Improve the stability of feed products | ||
| 4 | Advanced production control technology | Totally enclosed process, high degree of automatic control | Safe and stable quality |
| 5 | Advanced quality control technology | Establish and improve scientific and advanced analytical methods and control means for detecting factors affecting product quality, such as acid-soluble protein, molecular weight distribution, amino acids and chelating rate | Ensure quality, ensure efficiency and improve efficiency |
Part 7 Competitor Comparison
Standard VS Standard
Comparison of peptide distribution and chelation rate of products
| Sustar's products | Proportion of small peptides（180-500） | Zinpro's products | Proportion of small peptides（180-500） |
| AA-Cu | ≥74% | AVAILA-Cu | 78% |
| AA-Fe | ≥48% | AVAILA-Fe | 59% |
| AA-Mn | ≥33% | AVAILA-Mn | 53% |
| AA-Zn | ≥37% | AVAILA-Zn | 56% |
| Sustar's products | Chelation rate | Zinpro's products | Chelation rate |
| AA-Cu | 94.8% | AVAILA-Cu | 94.8% |
| AA-Fe | 95.3% | AVAILA-Fe | 93.5% |
| AA-Mn | 94.6% | AVAILA-Mn | 94.6% |
| AA-Zn | 97.7% | AVAILA-Zn | 90.6% |
The ratio of small peptides of Sustar is slightly lower than that of Zinpro, and the chelation rate of Sustar's products is slightly higher than that of Zinpro's products.
Comparison of the content of 17 amino acids in different products
| Name of amino acids | Sustar's Copper Amino Acid Chelate Feed Grade | Zinpro's AVAILA copper | Sustar's Ferrous Amino Acid C helate Feed Grade | Zinpro's AVAILA iron | Sustar's Manganese Amino Acid Chelate Feed Grade | Zinpro's AVAILA manganese | Sustar's Zinc Amino Acid Chelate Feed Grade | Zinpro's AVAILA zinc |
| aspartic acid (%) | 1.88 | 0.72 | 1.50 | 0.56 | 1.78 | 1.47 | 1.80 | 2.09 |
| glutamic acid (%) | 4.08 | 6.03 | 4.23 | 5.52 | 4.22 | 5.01 | 4.35 | 3.19 |
| Serine (%) | 0.86 | 0.41 | 1.08 | 0.19 | 1.05 | 0.91 | 1.03 | 2.81 |
| Histidine (%) | 0.56 | 0.00 | 0.68 | 0.13 | 0.64 | 0.42 | 0.61 | 0.00 |
| Glycine (%) | 1.96 | 4.07 | 1.34 | 2.49 | 1.21 | 0.55 | 1.32 | 2.69 |
| Threonine (%) | 0.81 | 0.00 | 1.16 | 0.00 | 0.88 | 0.59 | 1.24 | 1.11 |
| Arginine (%) | 1.05 | 0.78 | 1.05 | 0.29 | 1.43 | 0.54 | 1.20 | 1.89 |
| Alanine (%) | 2.85 | 1.52 | 2.33 | 0.93 | 2.40 | 1.74 | 2.42 | 1.68 |
| Tyrosinase (%) | 0.45 | 0.29 | 0.47 | 0.28 | 0.58 | 0.65 | 0.60 | 0.66 |
| Cystinol (%) | 0.00 | 0.00 | 0.09 | 0.00 | 0.11 | 0.00 | 0.09 | 0.00 |
| Valine (%) | 1.45 | 1.14 | 1.31 | 0.42 | 1.20 | 1.03 | 1.32 | 2.62 |
| Methionine (%) | 0.35 | 0.27 | 0.72 | 0.65 | 0.67 | 0.43 | January 0.75 | 0.44 |
| Phenylalanine (%) | 0.79 | 0.41 | 0.82 | 0.56 | 0.70 | 1.22 | 0.86 | 1.37 |
| Isoleucine (%) | 0.87 | 0.55 | 0.83 | 0.33 | 0.86 | 0.83 | 0.87 | 1.32 |
| Leucine (%) | 2.16 | 0.90 | 2.00 | 1.43 | 1.84 | 3.29 | 2.19 | 2.20 |
| Lysine (%) | 0.67 | 2.67 | 0.62 | 1.65 | 0.81 | 0.29 | 0.79 | 0.62 |
| Proline (%) | 2.43 | 1.65 | 1.98 | 0.73 | 1.88 | 1.81 | 2.43 | 2.78 |
| Total amino acids (%) | 23.2 | 21.4 | 22.2 | 16.1 | 22.3 | 20.8 | 23.9 | 27.5 |
Overall, the proportion of amino acids in Sustar's products is higher than that in Zinpro's products.
Part 8 Effects of use
Effects of different sources of trace minerals on the production performance and egg quality of laying hens in the late laying period
Production Process
- Targeted chelation technology
- Shear emulsification technology
- Pressure spray & drying technology
- Refrigeration & dehumidification technology
- Advanced environmental control technology
Appendix A: Methods for the Determination of relative molecular mass distribution of peptides
Adoption of standard: GB/T 22492-2008
1 Test Principle:
It was determined by high performance gel filtration chromatography. That is to say, using porous filler as stationary phase, based on the difference in the relative molecular mass size of the sample components for separation, detected at the peptide bond of the ultraviolet absorption wavelength of 220nm, using the dedicated data processing software for the determination of relative molecular mass distribution by gel filtration chromatography (i.e., the GPC software), the chromatograms and their data were processed, calculated to get the size of the relative molecular mass of the soybean peptide and the distribution range.
2. Reagents
The experimental water should meet the specification of secondary water in GB/T6682, the use of reagents, except for special provisions, are analytically pure.
2.1 Reagents include acetonitrile (chromatographically pure), trifluoroacetic acid (chromatographically pure),
2.2 Standard substances used in the calibration curve of relative molecular mass distribution: insulin, mycopeptides, glycine-glycine-tyrosine-arginine, glycine-glycine-glycine
3 Instrument and equipment
3.1 High Performance Liquid Chromatograph (HPLC): a chromatographic workstation or integrator with a UV detector and GPC data processing software.
3.2 Mobile phase vacuum filtration and degassing unit.
3.3 Electronic balance: graduated value 0.000 1g.
4 Operating steps
4.1 Chromatographic conditions and system adaptation experiments (reference conditions)
4.1.1 Chromatographic column: TSKgelG2000swxl300 mm×7.8 mm (inner diameter) or other gel columns of the same type with similar performance suitable for the determination of proteins and peptides.
4.1.2 Mobile phase: Acetonitrile + water + trifluoroacetic acid = 20 + 80 + 0.1.
4.1.3 Detection wavelength: 220 nm.
4.1.4 Flow rate: 0.5 mL/min.
4.1.5 Detection time: 30 min.
4.1.6 Sample injection volume: 20μL.
4.1.7 Column temperature: room temperature.
4.1.8 In order to make the chromatographic system meet the detection requirements, it was stipulated that under the above chromatographic conditions, the gel chromatographic column efficiency, i.e., the theoretical number of plates (N), was not less than 10000 calculated on the basis of the peaks of the tripeptide standard (Glycine-Glycine-Glycine).
4.2 Production of relative molecular mass standard curves
The above different relative molecular mass peptide standard solutions with a mass concentration of 1 mg / mL were prepared by mobile phase matching, mixed in a certain proportion, and then filtered through an organic phase membrane with the pore size of 0.2 μm~0.5 μm and injected into the sample, and then the chromatograms of the standards were obtained. Relative molecular mass calibration curves and their equations were obtained by plotting the logarithm of relative molecular mass against retention time or by linear regression.
4.3 Sample treatment
Accurately weigh 10mg of sample in a 10mL volumetric flask, add a little mobile phase, ultrasonic shaking for 10min, so that the sample is fully dissolved and mixed, diluted with mobile phase to the scale, and then filtered through an organic phase membrane with a pore size of 0.2μm~0.5μm, and the filtrate was analyzed according to the chromatographic conditions in A.4.1.
5. Calculation of relative molecular mass distribution
After analyzing the sample solution prepared in 4.3 under the chromatographic conditions of 4.1, the relative molecular mass of the sample and its distribution range can be obtained by substituting the chromatographic data of the sample into the calibration curve 4.2 with GPC data processing software. The distribution of the relative molecular masses of the different peptides can be calculated by the peak area normalization method, according to the formula: X=A/A total×100
In the formula: X - The mass fraction of a relative molecular mass peptide in the total peptide in the sample, %;
A - Peak area of a relative molecular mass peptide;
Total A - the sum of the peak areas of each relative molecular mass peptide, calculated to one decimal place.
6 Repeatability
The absolute difference between two independent determinations obtained under conditions of repeatability shall not exceed 15% of the arithmetic mean of the two determinations.
Appendix B: Methods for the Determination of Free Amino Acids
Adoption of standard: Q/320205 KAVN05-2016
1.2 Reagents and materials
Glacial acetic acid: analytically pure
Perchloric acid: 0.0500 mol/L
Indicator: 0.1% crystal violet indicator (glacial acetic acid)
2. Determination of free amino acids
The samples were dried at 80°C for 1 hour.
Place the sample in a dry container to cool naturally to room temperature or cool down to a usable temperature.
Weigh approximately 0.1 g of sample (accurate to 0.001 g) into a 250 mL dry conical flask.
Quickly proceed to the next step to avoid the sample from absorbing ambient moisture
Add 25 mL of glacial acetic acid and mix well for no more than 5 min.
Add 2 drops of crystal violet indicator
Titrate with 0.0500 mol / L (±0.001) standard titration solution of perchloric acid until the solution changes from purple to the end point.
Record the volume of standard solution consumed.
Carry out the blank test at the same time.
3. Calculation and results
The free amino acid content X in the reagent is expressed as a mass fraction (%) and is calculated according to the formula: X = C × (V1-V0) × 0.1445/M × 100%, in tne formula:
C - Concentration of standard perchloric acid solution in moles per liter (mol/L)
V1 - Volume used for titration of samples with standard perchloric acid solution, in milliliters (mL).
Vo - Volume used for titration blank with standard perchloric acid solution, in milliliters (mL);
M - Mass of the sample, in grams (g ).
0.1445: Average mass of amino acids equivalent to 1.00 mL of standard perchloric acid solution [c (HClO4) = 1.000 mol / L].
Appendix C: Methods for the Determination of Sustar's chelation rate
Adoption of standards: Q/70920556 71-2024
1. Determination principle (Fe as an example)
Amino acid iron complexes have very low solubility in anhydrous ethanol and free metal ions are soluble in anhydrous ethanol, the difference in solubility between the two in anhydrous ethanol was utilized to determine the chelation rate of amino acid iron complexes.
2. Reagents & Solutions
Anhydrous ethanol; the rest is the same as clause 4.5.2 in GB/T 27983-2011.
3. Steps of analysis
Do two trials in parallel. Weigh 0.1g of the sample dried at 103±2℃ for 1 hour, accurate to 0.0001g, add 100mL of anhydrous ethanol to dissolve, filter, filter residue washed with 100mL of anhydrous ethanol for at least three times, then transfer the residue into a 250mL conical flask, add 10mL of sulfuric acid solution according to clause 4.5.3 in GB/T27983-2011, and then perform the following steps according to clause 4.5.3 “Heat to dissolve and then let cool” in GB/T27983-2011. Carry out the blank test at the same time.
4. Determination of total iron content
4.1 The principle of determination is the same as clause 4.4.1 in GB/T 21996-2008.
4.2. Reagents & Solutions
4.2.1 Mixed acid: Add 150mL of sulfuric acid and 150mL of phosphoric acid to 700mL of water and mix well.
4.2.2 Sodium diphenylamine sulfonate indicator solution: 5g/L, prepared according to GB/T603.
4.2.3 Cerium sulfate standard titration solution: concentration c [Ce (SO4) 2] = 0.1 mol/L, prepared according to GB/T601.
4.3 Steps of analysis
Do two trials in parallel. Weigh 0.1g of sample, accurate to 020001g, place in a 250mL conical flask, add 10mL of mixed acid, after dissolution, add 30ml of water and 4 drops of sodium dianiline sulfonate indicator solution, and then perform the following steps according to clause 4.4.2 in GB/T21996-2008. Carry out the blank test at the same time.
4.4 Representation of results
The total iron content X1 of the amino acid iron complexes in terms of mass fraction of iron, the value expressed in %, was calculated according to formula (1):
X1=(V-V0)×C×M×10-3×100
In the formula: V - volume of cerium sulfate standard solution consumed for titration of test solution, mL;
V0 - cerium sulfate standard solution consumed for titration of blank solution, mL;
C - Actual concentration of cerium sulfate standard solution, mol/L
5. Calculation of iron content in chelates
The iron content X2 in the chelate in terms of the mass fraction of iron, the value expressed in %, was calculated according to the formula: x2 = ((V1-V2) × C × 0.05585)/m1 × 100
In the formula: V1 - volume of cerium sulfate standard solution consumed for titration of test solution, mL;
V2 - cerium sulfate standard solution consumed for titration of blank solution, mL;
C - Actual concentration of cerium sulfate standard solution, mol/L;
0.05585 - mass of ferrous iron expressed in grams equivalent to 1.00 mL of cerium sulfate standard solution C[Ce(SO4)2.4H20] = 1.000 mol/L.
m1-Mass of the sample, g. Take the arithmetic mean of the parallel determination results as the determination results, and the absolute difference of the parallel determination results is not more than 0.3%.
6. Calculation of chelation rate
Chelation rate X3, the value expressed in %, X3 = X2/X1 × 100
Appendix C: Methods for the Determination of Zinpro's chelation rate
Adoption of standard: Q/320205 KAVNO7-2016
1. Reagents and materials
a) Glacial acetic acid: analytically pure; b) Perchloric acid: 0.0500mol/L; c) Indicator: 0.1% crystal violet indicator (glacial acetic acid)
2. Determination of free amino acids
2.1 The samples were dried at 80°C for 1 hour.
2.2 Place the sample in a dry container to cool naturally to room temperature or cool down to a usable temperature.
2.3 Weigh approximately 0.1 g of sample (accurate to 0.001 g) into a 250 mL dry conical flask
2.4 Quickly proceed to the next step to avoid the sample from absorbing ambient moisture.
2.5 Add 25mL of glacial acetic acid and mix well for no more than 5min.
2.6 Add 2 drops of crystal violet indicator.
2.7 Titrate with 0.0500mol/L (±0.001) standard titration solution of perchloric acid until the solution changes from purple to green for 15s without changing color as the end point.
2.8 Record the volume of standard solution consumed.
2.9 Carry out the blank test at the same time.
3. Calculation and results
The free amino acid content X in the reagent is expressed as a mass fraction (%), calculated according to formula (1): X=C×(V1-V0) ×0.1445/M×100%...... .......(1)
In the formula: C - concentration of standard perchloric acid solution in moles per liter (mol/L)
V1 - Volume used for titration of samples with standard perchloric acid solution, in milliliters (mL).
Vo - Volume used for titration blank with standard perchloric acid solution, in milliliters (mL);
M - Mass of the sample, in grams (g ).
0.1445 - Average mass of amino acids equivalent to 1.00 mL of standard perchloric acid solution [c (HClO4) = 1.000 mol / L].
4. Calculation of chelation rate
The chelation rate of the sample is expressed as mass fraction (%), calculated according to formula (2): chelation rate = (total amino acid content - free amino acid content)/total amino acid content×100%.
Post time: Sep-17-2025
